single cell genome amplification accelerates identification of the apratoxin biosynthetic pathway from a complex microbial assemblage单细胞基因组扩增的加速识别apratoxin从一个复杂的微生物组合生物合成途径.pdf

Single Cell Genome Amplification Accelerates Identification of the Apratoxin Biosynthetic Pathway from a Complex Microbial Assemblage 1 2 3 1,4 1 Rashel V. Grindberg , Thomas Ishoey , Dumitru Brinza , Eduardo Esquenazi , R. Cameron Coates , 5 1 4,5 3 2 Wei-ting Liu , Lena Gerwick , Pieter C. Dorrestein , Pavel Pevzner , Roger Lasken , William H. Gerwick1,4* 1 Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California San Diego, La Jolla, California, United States of America, 2 J. Craig Venter Institute, San Diego, California, United States of America, 3 Department of Computer Science and Engineering, Center for Algorithmic and Systems Biology, University of California San Diego, La Jolla, California, United States of America, 4 Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, United States of America, 5 Departments of Chemistry and Biochemistry, University of California San Diego, La Jolla, California, United States of America Abstract Filamentous marine cyanobacteria are extraordinarily rich sources of structurally novel, biomedically relevant natural products. To understand their biosynthetic origins as well as produce increased supplies and analog molecules, access to the clustered biosynthetic genes that encode for the assembly enzymes is necessary. Complicating these efforts is the universal presence of heterotrophic bacteria in the cell wall and sheath material of cyanobacter