bivalirudin in combination with heparin to control mesenchymal cell procoagulant activitybivalirudin结合肝素控制间充质细胞促凝血的活动.pdf

Bivalirudin in Combination with Heparin to Control Mesenchymal Cell Procoagulant Activity 1,2 1,2 3 4 1 Xavier Stephenne *, Emanuele Nicastro , Stephane Eeckhoudt , Cedric Hermans , Omar Nyabi , 1 1 1,2 Catherine Lombard , Mustapha Najimi , Etienne Sokal ´ ´ ´ 1 Universite Catholique de Louvain, Institut de Recherche Experimentale et Clinique, Unite de Recherche PEDI, Brussels, Belgium, 2 Cliniques Universitaires Saint Luc, ´ ´ ´ ´ ´ Service de Gastroenterologie et Hepatologie Pediatrique, Brussels, Belgium, 3 Cliniques Universitaires Saint Luc, Laboratoire d’Hematologie–Hemostase, Brussels, Belgium, ´ ´ ´ 4 Cliniques Universitaires Saint Luc, Service d’Hematologie, Unite d’Hemostase-Thrombose, Brussels, Belgium Abstract Islet and hepatocyte transplantation are associated with tissue factor-dependent activation of coagulation which elicits instant blood mediated inflammatory reaction, thereby contributing to a low rate of engraftment. The aim of this study was i) to evaluate the procoagulant activity of human adult liver-derived mesenchymal progenitor cells (hALPCs), ii) to compare it to other mesenchymal cells of extra-hepatic (bone marrow mesenchymal stem cells and skin fibroblasts) or liver origin (liver myofibroblasts), and iii) to determine the ways this activity could be modulated. Usin