the active ingredients of jiang-zhi-ning study of the nelumbo nucifera alkaloids and their main bioactive metabolites莲属椰子的jiang-zhi-ning研究活性成分生物碱及其主要生物活性代谢物.pdf

Molecules 2012, 17, 9855-9867; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article The Active Ingredients of Jiang-Zhi-Ning: Study of the Nelumbo nucifera Alkaloids and Their Main Bioactive Metabolites Jianxin Chen †, Xueling Ma †, Kuo Gao, Yong Wang, Huihui Zhao, Hao Wu, Juan Wang, Hua Xie, Yulin OuYang, Liangtao Luo, Shuzhen Guo, Jing Han, Bing Liu and Wei Wang * Beijing University of Chinese Medicine, 11 Bei San Huan Dong Lu, ChaoYang District, Beijing 100029, China † These authors contributed equally to this work. * Author to whom correspondence should be addressed; E-Mail: wangwei@; Tel.: +86-10-6428-6508; Fax: +86-10-6428-6283. Received: 15 June 2012; in revised form: 12 July 2012 / Accepted: 17 July 2012 / Published: 16 August 2012 Abstract: The object of this study was to identify the major active ingredients of the Chinese Traditional Medicine Jiang-Zhi-Ning (JZN) based on the high performance liquid chromatography (HPLC) profiles of plasma samples obtained from beagle dogs at different times after intragastric administration of JZN, crude JZN extracts, different extracted fractions, different subfractions of the active fraction and different isolated ingredients. 2-Hydroxy-1-methoxyaporphin (2H1M), an alkaloid from Nelumbo nucifera, one of the herbs that make up JZN, was identified as the constituent showing the major pharmacodynamic effect. The major metabolites of 2H1M were analyzed and identified as N-demethyl-2-hydroxy-1-methoxyapor