bacterial communities of two ubiquitous great barrier reef corals reveals both site- and species-specificity of common bacterial associates细菌社区两个无处不在的大堡礁的珊瑚揭示了这两个网站,常见的细菌associates的种特异性.pdf

Bacterial Communities of Two Ubiquitous Great Barrier Reef Corals Reveals Both Site- and Species-Specificity of Common Bacterial Associates 1,2,4 1,2 1 1,3 E. Charlotte E. Kvennefors *, Eugenia Sampayo , Tyrone Ridgway , Andrew C. Barnes , Ove Hoegh-Guldberg1,2,4 1 Centre for Marine Studies, The University of Queensland, Brisbane, Queensland, Australia, 2 Australian Research Council Centre of Excellence for Coral Reef Studies, The University of Queensland, Brisbane, Queensland, Australia, 3 School of Biological Sciences, The University of Queensland, Brisbane, Queensland, Australia, 4 Global Change Institute, The University of Queensland, Brisbane, Queensland, Australia Abstract Background: Coral-associated bacteria are increasingly considered to be important in coral health, and altered bacterial community structures have been linked to both coral disease and bleaching. Despite this, assessments of bacterial communities on corals rarely apply sufficient replication to adequately describe the natural variability. Replicated data such as these are crucial in determining potential roles of bacteria on coral. Methodology/Principal Findings: Denaturing Gradient Gel Electrophoresis (DGGE) of the V3 region of the 16S ribosomal DNA was used in a highly replicated approach to analyse bacterial communities on both healthy and diseased corals. Although site-specific variations in the bacterial communities of healthy corals were present, host species-specific bacterial associates within a distinct cluster of gamma-proteobacteria could be identified, which are potentially linked to coral health. Corals affected by ‘‘White Syndrome’’ (WS) underwent pronoun