α-synuclein reactive antibodies as diagnostic biomarkers in blood sera of parkinsons disease patientsα-synuclein活性抗体血血清诊断生物标记的帕金森症患者.pdf

a-Synuclein Reactive Antibodies as Diagnostic Biomarkers in Blood Sera of Parkinson’s Disease Patients 1 2 3 3 4 Kiran Yanamandra , Marina A. Gruden , Vida Casaite , Rolandas Meskys , Lars Forsgren , Ludmilla A. Morozova-Roche1* ˚ ˚ 1 Department of Medical Biochemistry and Biophysics, Umea University, Umea, Sweden, 2 P.K. Anokhin Institute of Normal Physiology, RAMS Moscow, Russia, 3 Departments of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania, 4 Department of Pharmacology and Clinical ˚ ˚ Neuroscience, Umea University, Umea, Sweden Abstract Background: Auto-antibodies with specificity to self-antigens have been implicated in a wide variety of neurological diseases, including Parkinson’s (PD) and Alzheimer’s diseases, being sensitive indicators of neurodegeneration and focus for disease prevention. Of particular interest are the studies focused on the auto-immune responses to amyloidogenic proteins associated with diseases and their applications in therapeutic treatments such as vaccination with amyloid antigens and antibodies in PD, Alzheimer’s disease and potentially other neurodegeneration ailments. Methodology/Principal Findings: Generated auto-antibodies towards the major amyloidogenic protein involved in PD Lewy bodies – a-synuclein and its amyloid oligomers and fibrils were measured in the blood sera of early and late PD patients and controls by using ELISA, Western blot and Biacore surface plasmon resonance. We found significantly higher antibody levels towards monomeric a-synuclein in the blood sera of PD patients co