conserved cdc20 cell cycle functions are carried out by two of the five isoforms in arabidopsis thaliana守恒cdc20细胞周期函数是由两个拟南芥的五个亚型.pdf

Conserved CDC20 Cell Cycle Functions Are Carried out by Two of the Five Isoforms in Arabidopsis thaliana ´ 1,2. 1. 1 2 1 1 Zoltan Kevei , Mikhail Baloban , Olivier Da Ines , Hilda Tiricz , Alexandra Kroll , Krzysztof Regulski , Peter Mergaert1, Eva Kondorosi1,2* ´ ´ ´ 1 Institut des Sciences du Vegetal, Centre National de la Recherche Scientifique, Unite Propre de Recherche 2355, Gif-sur-Yvette, France, 2 Institute for Plant Genomics, Human Biotechnology and Bioenergy, Bay Zoltan Foundation for Applied Research, Szeged, Hungary Abstract Background: The CDC20 and Cdh1/CCS52 proteins are substrate determinants and activators of the Anaphase Promoting Complex/Cyclosome (APC/C) E3 ubiquitin ligase and as such they control the mitotic cell cycle by targeting the degradation of various cell cycle regulators. In yeasts and animals the main CDC20 function is the destruction of securin and mitotic cyclins. Plants have multiple CDC20 gene copies whose functions have not been explored yet. In Arabidopsis thaliana there are five CDC20 isoforms and here we aimed at defining their contribution to cell cycle regulation, substrate selectivity and plant development. Methodology/Principal Findings: Studying the gene structure and phylogeny of plant CDC20s, the expression of the five AtCDC20 gene copies and their interactions with the APC/C subunit APC10, the CCS52 proteins, components of the mitotic checkpoint complex (MCC) and mitotic cyclin substrates, conserved CDC20 functions could be assigned for AtCDC20.1 and AtCDC20.2. The other three intron-less