deciduous trees and the application of universal dna barcodes a case study on the circumpolar fraxinus落叶树木和普遍的应用在环极fraxinus dna条形码的案例研究.pdf

Deciduous Trees and the Application of Universal DNA Barcodes: A Case Study on the Circumpolar Fraxinus 1,2,3. 1,2,3,4 . 5 {6 4 Mariangela Arca , Damien Daniel Hinsinger * , Corinne Cruaud , Annie Tillier , Jean Bousquet , Nathalie Frascaria-Lacoste1,2,3* ´ 1 Universite Paris Sud, UMR 8079, Orsay, France, 2 Centre national de la recherche scientifique, UMR 8079, Orsay, France, 3 AgroParisTech, UMR 8079, Orsay, France, ´ ` ´ ˆ ´ ´ ´ ´ 4 Chaire de recherche du Canada en genomique forestiere et environnementale, Centre d’etude de la foret, Universite Laval, Quebec, Quebec, Canada, 5 Genoscope, ´ ´ ´ ´ ´ ´ ´ Centre national de sequenc¸age, Evry, France, 6 Departement systematique et evolution and Service de systematique moleculaire, Museum national d’histoire naturelle, Paris, France Abstract The utility of DNA barcoding for identifying representative specimens of the circumpolar tree genus Fraxinus (56 species) was investigated. We examined the genetic variability of several loci suggested in chloroplast DNA barcode protocols such as matK, rpoB, rpoC1 and trnH-psbA in a large worldwide sample of Fraxinus species. The chloroplast intergenic spacer rpl32- trnL was further assessed in search for a potentially variable and useful locus. The results of the study suggest that the proposed cpDNA loci, alone or in combination, cannot fully discriminate among species beca