sulfated polysaccharides in marine sponges extraction methods and anti-hiv activity在海洋硫酸多糖海绵提取方法和抗艾滋病活动.pdf

Mar. Drugs 2011, 9, 139-153; doi:10.3390/md9010139 OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Sulfated Polysaccharides in Marine Sponges: Extraction Methods and Anti-HIV Activity Ana I. S. Esteves 1,*, Marisa Nicolai 2, Madalena Humanes 3 and Joao Goncalves 4 1 Centre of Marine Sciences, University of Algarve, Gambelas Campus, 8005-139 Faro, Portugal 2 Oral and Biomedical Sciences Research Unit, Faculty of Dentistry, University of Lisbon, 1649-003 Lisbon, Portugal; E-Mail: mhnicolai@ 3 Centre of Chemistry and Biochemistry, Faculty of Sciences, University of Lisbon, Building C8, Campo Grande, 1749-016 Lisbon, Portugal; E-Mail: mmhumanes@fc.ul.pt 4 Research Unit for Retrovirus and Associated Infections, Centre of Molecular Pathogenesis, Faculty of Farmacy, University of Lisbon, Avenida das Forcas Armadas, 1649-019 Lisbon, Portugal; E-Mail: joao.goncalves@ff.ul.pt * Author to whom correspondence should be addressed; E-Mail: aidsesteves@; Tel.: +351-289-800-051; Fax: +351-289-800-051. Received: 6 December 2010; in revised form: 9 January 2011 / Accepted: 20 January 2011 / Published: 24 January 2011 Abstract: The extraction, fractionation and HIV-1 inhibition potential of polysaccharides extracted from three species of marine sponges, Erylus discophorus, Cliona celata and Stelletta sp., collected in the Northeastern Atlantic, is presented in this work. The anti-HIV activity of 23 polysaccharide pellets and three crude extracts was tested. Crude extracts prepared from