association of hiv diversity and survival in hiv-infected ugandan infants协会在艾滋病毒感染hiv病毒的多样性和生存乌干达婴儿.pdf

Association of HIV Diversity and Survival in HIV-Infected Ugandan Infants 1 2 3,4 2 1 1 Maria M. James , Lei Wang , Philippa Musoke , Deborah Donnell , Jessica Fogel , William I. Towler , 1 3,4 1 1 Leila Khaki , Clemensia Nakabiito , J. Brooks Jackson , Susan H. Eshleman * 1Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America, 3 Makerere University-Johns Hopkins University Research Collaboration (MU-JHU), Baltimore, Maryland, United States of America, and Kampala, Uganda, 4 Makerere University School of Medicine, Kampala, Uganda Abstract Background: The level of viral diversity in an HIV-infected individual can change during the course of HIV infection, reflecting mutagenesis during viral replication and selection of viral variants by immune and other selective pressures. Differences in the level of viral diversity in HIV-infected infants may reflect differences in viral dynamics, immune responses, or other factors that may also influence HIV disease progression. We used a novel high resolution melting (HRM) assay to measure HIV diversity in Ugandan infants and examined the relationship between diversity and survival through 5 years of age. Methods: Plasma samples were obtained from 31 HIV-infected infants (HIVNET 012 trial). The HRM assay was used to measure diversity in two regions in the gag gene (Gag1 and Gag2) and one region in the pol gene (Pol). Results: HRM scores in all three reg