supercritical fluid extraction and ultra performance liquid chromatography of respiratory quinones for microbial community analysis in environmental and biological samples超临界流体萃取和超高液相色谱呼吸醌类微生物群落分析在环境和生物样品.pdf

Molecules 2012, 1 7, 2628-2642; doi:10.3390/molecule OPEN ACCESS molecules ISSN 1420-3049 /journal/molecules Article Supercritical Fluid Extraction and Ultra Performance Liquid Chromatography of Respiratory Quinones for Microbial Community Analysis in Environmental and Biological Samples Muhammad Hanif 1,2, Yoichi Atsuta 1, Koichi Fujie 3 and Hiroyuki Daimon 1,* 1 Department of Environmental and Life Sciences, Toyohashi University of Technology, Aichi 441-8580, Japan; E-Mails: hanif@water.ens.tut.ac.jp (M.H.); atsuta@water.ens.tut.ac.jp (Y.A.) 2 Center for Energy Resources Development, Agency for the Assessment and Application of Technology, Jakarta 10340, Indonesia 3 Graduate School of Environment and Information Sciences, Yokohama National University, Kanagawa 240-8501, Japan; E-Mail: fujie@ynu.ac.jp * Author to whom correspondence should be addressed; E-Mail: daimon@ens.tut.ac.jp; Tel.: +81-532-44-6905; Fax: +81-532-44-6905. Received: 16 December 2011; in revised form: 23 February 2012 / Accepted: 28 February 2012 / Published: 5 March 2012 Abstract: Microbial community structure plays a significant role in environmental assessment and animal health management. The development of a superior analytical strategy for the characterization of microbial community structure is an ongoing challenge. In this study, we developed an effective supercritical fluid extraction (SFE) and ultra performance liquid chromatography (UPLC) method for the