the 5′utr-intron of the gladiolus polyubiquitin promoter gubq1 enhances translation efficiency in gladiolus and arabidopsis的5u2032utr-intron剑兰polyubiquitin发起人gubq1提高翻译效率在剑兰和拟南芥.pdf

Kamo et al. BMC Plant Biology 2012, 12:79 /1471-2229/12/79 RESEARCH ARTICLE Open Access ′ The 5 UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis 1† 2 2 2* Kathryn Kamo , Ah-Young Kim , Se Hee Park and Young Hee Joung Abstract Background: There are many non-cereal monocots of agronomic, horticultural, and biofuel importance. Successful transformation of these species requires an understanding of factors controlling expression of their genes. Introns have been known to affect both the level and tissue-specific expression of genes in dicots and cereal monocots, but there have been no studies on an intron isolated from a non-cereal monocot. This study characterizes the levels of GUS expression and levels of uidA mRNA that code for β-glucuronidase (GUS) expression in leaves of Gladiolus and Arabidopsis using GUBQ1, a polyubiquitin promoter with a 1.234 kb intron, isolated from the non-cereal monocot Gladiolus, and an intronless version of this promoter. Results: Gladiolus and Arabidopsis were verified by Southern hybridization to be transformed with the uidA gene that was under control of either the GUBQ1 promoter (1.9 kb), a 5′ GUBQ1 promoter missing its 1.234 kb intron (0.68 kb), or the CaMV 35 S promoter. Histochemical staining showed that GUS was expressed throughout leaves and roots of Gladiolus and Arabidopsis with the 1.9 kb GUBQ1 promoter. GUS expression was significantly decreased ′ in Gladiolus and abolished in Arabidopsis when the 5 UTR-intron was absent. In Arabidopsis and Gladiolus, the