the effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in arabidopsis thaliana气候变暖对叶片功能性状的影响实验,在拟南芥叶结构和生物化学.pdf

Jin et al. BMC Plant Biology 2011, 11:35 /1471-2229/11/35 RESEARCH ARTICLE Open Access The effect of experimental warming on leaf functional traits, leaf structure and leaf biochemistry in Arabidopsis thaliana 1,4† 3,4† 4 4 4 4 4 Biao Jin , Li Wang , Jing Wang , Ke-Zhen Jiang , Yang Wang , Xiao-Xue Jiang , Cheng-Yang Ni , Yu-Long Wang5, Nian-Jun Teng2* Abstract Background: The leaf is an important plant organ, and how it will respond to future global warming is a question that remains unanswered. The effects of experimental warming on leaf photosynthesis and respiration acclimation has been well studied so far, but relatively little information exists on the structural and biochemical responses to warming. However, such information is very important to better understand the plant responses to global warming. Therefore, we grew Arabidopsis thaliana at the three day/night temperatures of 23/18°C (ambient temperature), 25.5/20.5°C (elevated by 2.5°C) and 28/23°C (elevated by 5°C) to simulate the middle and the upper projected warming expected within the 21st century for this purpose. Results: The 28/23°C treatment significantly reduced the life span, total biomass and total weight of seeds compared with the other two temperatures. Among the three temperature regimes, the concentrations of starch, chlorophyll, and proline were the lowest at 28/23°C, whereas the total weight of seeds, concentrations of chlorophyll and proline, stomatal density (SD), stomatal conductance (gs), net CO2 assimilation rate (A) and transpiration rate (E) were the highest at 25.5/20.5°C. Furthermore, the number of chloroplasts per cell and mitochondrial size were highest at 25.5/20.5°C and lowest at 28/23°C. Conclusions: The condi