双水相萃取体系及其在蛋白质和酶分离中的应用..doc

 PEG/无机盐双水相萃取体系及其在蛋白质和酶分离中的应用 摘 要 双水相萃取技术是一种新型液-液萃取技术，已应用于生物活性物质的分离。本文阐述了双水相萃取技术的基本原理与特点，并对其中的PEG（聚乙二醇）/无机盐双水相萃取体系及其在蛋白质和酶分离中的应用进行了综述。对双水相萃取技术存在的问题与发展前景进行了简要论述。 关键词 双水相萃取；分离；蛋白质；酶；聚乙二醇 the polyethylene glycol/salt aqueous two-phase system and its application to partition and purification of proteins and enzymes ABSTRACT Aqueous two-phase extraction technique is a new-type liquid-liquid extraction, which has been applied to biological activity compounds. The basic principles and features of aqueous two- phase extraction technique were illustrated, and the polyethylene glycol/salt aqueous two-phase system and its application to partition and purification of proteins and enzymes were summarized. Its unresolved problems and directions in the future were also pointed out. KEY WORDS aqueous two-phase extraction; partition; proteins; enzymes; polyethylene glycol 目 录 中文摘要………………………………………………………………………………………Ⅰ 英文摘要………………………………………………………………………………………Ⅱ 目录……………………………………………………………………………………………Ⅲ 1前言……………………………………………………………………………………………1 2………………………………………………………………………2 2.1双水相的形成…………………………………………………………………………2 2.2双水相萃取的原理……………………………………………………………………2 2.3双水相萃取体系的分类…………………………………………………………………2 2.4双水相萃取的特点……………………………………………………………………2 3 PEG/无机盐双水相萃取体系…………………………………………………………………3 3.1 PEG/无机盐双水相萃取体系的制备……………………………………………………3 3.2 PEG/无机盐双水相萃取体系的分相规律………………………………………………4 3.3 PEG/无机盐双水相体系性能参数和影响因素…………………………………………5 4萃取条件优化……………………………………………………………………………6 4.1 PEG种类和浓度的选择………………………………………………………………6 4.2成相盐种类和浓度的选择………………………………………………………………8 4.3 pH的选择………………………………………………………………………………10 4.4外加电解种类和浓度的选择…………………………………………………………12 4.5其他影响因素的优化…………………………………………………………………12 5 PEG/无机盐双水相体系在蛋白质和酶分离中的应用………………………………………14 5.1蛋白质和酶的分离及评价参数………………………………………………14 5.2 PEG/无机盐双水相体系在蛋白质和酶分离中的应用……………………………15 5.3 PEG/无机盐双水相体系在生物工程中的分离流程………………………………20 6结语…………………………………………………………………………………21 参考文献………………………………………………………………………………………前言[1]，已经越来越受到人们的重视。 Beijeronck在1896年将琼脂水溶液与可溶性淀粉或明胶水溶液混合，发现了双水相现象。双水相萃取(Aqueou