基质鲎试剂盒使用说明书(含偶氮)[].doc

基质鲎试剂盒使用说明书(含偶氮)[1] Chromogenic substrate limulus test kit instructions (endpoint chromogenic method, containing even nitrided reagent) [purpose] chromogenic substrate Limulus amebocyte lysate (Chromogenic End-point Tachypleus Amebocyte Lysate), CE TAL is used for quantitative detection of bacterial endotoxin in vitro, and it is prohibited to enter the body in any way. [principle] Limulus amebocyte lysate is a freeze-dried product of blood cell lysate in Limulus amebocyte lysate of horseshoe crab It contains C factor, B factor, coagulation zymogen, coagulation protein and so on. Under suitable conditions (temperature) Bacterial endotoxin activates C factor, resulting in a series of enzymatic reactions, pH values and non interfering substances, Activate coagulase to form coagulase, coagulase decompose synthetic color matrix, make it decompose into Polypeptide and yellow para aniline (pNA, lambda max = 405nm). Within a certain period of time, pNAs The amount of endotoxin produced was positively correlated with the concentration of bacterial endotoxin, and the endotoxin concentration of the tested products could be quantified. At the same time, p-nitrophenol (pNA) can also be dyed with an azo reagent (Rose = max = 545nm), The interference of the color of the sample itself to the absorption peak at 405nm is avoided. [detection limit] two regions of 0.1EU/ml-1, EU/ml and 0.01EU/ml -0.1EU/ml can be detected according to different reaction time Inspection report of factory inspection (reaction time T 1 and T 2). [kit composition] 2 pieces of bacterial endotoxin products Limulus amebocyte lysate, 1.7ml/ branch, 2 branches Color matrix, 1.7ml/ branch, 2 branches Azo reagent 1, 10ml/ branch, 2 branches Azo reagent 2, 10ml/ branch, 2 branches Azo reagent 3, 10ml/ branch, 2 branches HCl (reaction terminator), 50ml/ bottle, 1 bottles Bacterial endotoxin test water, 50ml/ bottle, 2 bottles [storage] cool, best 2-8 degrees, avoid light storage. [usage] 1. materials and equipment 1