association of alpha b-crystallin genotypes with oral cancer susceptibility, survival, and recurrence in taiwanαb-crystallin协会与口腔癌易感性基因型,在台湾生存和复发.pdf

Association of Alpha B-Crystallin Genotypes with Oral Cancer Susceptibility, Survival, and Recurrence in Taiwan 1,2,3 1,2 1,4 1 1,5 Da-Tian Bau *, Chia-Wen Tsai , Cheng-Chieh Lin , Ru-Yin Tsai , Ming-Hsui Tsai * 1Terry Fox Cancer Research Laboratory, China Medical University Hospital, Taichung, Taiwan, 2 Graduate Institute of Basic Medical Science, China Medical University, Taichung, Taiwan, 3 Graduate Institute of Clinical Medical Science, China Medical University, Taichung, Taiwan, 4 Department of Family Medicine, China Medical University Hospital, Taichung, Taiwan, 5 Department of Otolaryngology, China Medical University Hospital, Taichung, Taiwan Abstract Background: Alpha B-crystallin (CRYAB) is a protein that functions as ‘‘molecular chaperone’’ in preserving intracellular architecture and cell membrane. Also, CRYAB is highly antiapoptotic. Abnormal CRYAB expression is a prognostic biomarker for oral cancer, while its genomic variations and the association with carcinogenesis have never been studied. Methodology/Finding: Therefore, we hypothesized that CRYAB single nucleotide polymorphisms may be associated with oral cancer risk. In this hospital-based study, the association of CRYAB A-1215G (rs2228387), C-802G (rs14133) and intron2 (rs2070894) polymorphisms with oral cancer in a Taiwan population was investigated. In total, 496 oral cancer patients and 992 age- and gender-matched healthy controls were genotyped and analyzed. A significantly different frequency distribution was found in CRYAB C-802G genotypes, but not in A-1215G and intron2 genotypes, between the oral cancer and control groups. The CRYAB C-802G G allele conferred an increased risk of oral cancer (