comparative study of human and mouse postsynaptic proteomes finds high compositional conservation and abundance differences for key synaptic proteins人类和小鼠突触后蛋白质组的比较研究发现高成分保护和关键突触蛋白的丰度差异.pdf

Comparative Study of Human and Mouse Postsynaptic Proteomes Finds High Compositional Conservation and Abundance Differences for Key Synaptic Proteins ` ´ 1,2,3 4 2,3 2,3 4 Alex Bayes *, Mark O. Collins , Mike D. R. Croning , Louie N. van de Lagemaat , Jyoti S. Choudhary , Seth G. N. Grant2,3* 1 Molecular Physiology of the Synapse Laboratory, Institut de Recerca de l’Hospital de la Santa Creu i Sant Pau, UAB, Barcelona, Catalonia, Spain, 2 Genes to Cognition Programme, Wellcome Trust Sanger Institute, Genome Campus, Hinxton, Cambridgeshire, United Kingdom, 3 Department of Clinical Neuroscience. School of Molecular and Clinical Medicine, University of Edinburgh, Edinburgh, United Kingdom, 4 Proteomic Mass Spectrometry, The Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, United Kingdom Abstract Direct comparison of protein components from human and mouse excitatory synapses is important for determining the suitability of mice as models of human brain disease and to understand the evolution of the mammalian brain. The postsynaptic density is a highly complex set of proteins organized into molecular networks that play a central role in behavior and disease. We report the first direct comparison of the proteome of triplicate isolates of mouse and human cortical postsynaptic densities. The mouse postsynaptic density comprised 1556 proteins and the human one 1461. A large compositional overlap was observed; more than 70% of human postsynaptic density proteins were also observed in the mouse postsynaptic density. Quantitative analysis of postsynaptic density components in both species indicates a broadly similar profile of abundance