de novo transcriptome sequencing in anopheles funestus using illumina rna-seq technology新创转录组测序在使用illumina公司rna-seq技术按蚊.pdf

De Novo Transcriptome Sequencing in Anopheles funestus Using Illumina RNA-Seq Technology 1 2 2 ´ 2 Jacob E. Crawford *, Wamdaogo M. Guelbeogo , Antoine Sanou , Alphonse Traore , Kenneth D. 3,4 2 1 Vernick , N’Fale Sagnon , Brian P. Lazzaro 1 Department of Entomology, Cornell University, Ithaca, New York, United States of America, 2 Centre National de Recherche et de Formation sur le Paludisme, Ouagadougou, Burkina Faso, 3 Department of Parasitology and Mycology, Centre National de la Recherche Scientifique Unit URA3012: Hosts, Vectors and Infectious Agents, Institut Pasteur, Paris, France, 4 Department of Microbiology, University of Minnesota, Saint Paul, Minnesota, United States of America Abstract Background: Anopheles funestus is one of the primary vectors of human malaria, which causes a million deaths each year in sub-Saharan Africa. Few scientific resources are available to facilitate studies of this mosquito species and relatively little is known about its basic biology and evolution, making development and implementation of novel disease control efforts more difficult. The An. funestus genome has not been sequenced, so in order to facilitate genome-scale experimental biology, we have sequenced the adult female transcriptome of An. funestus from a newly founded colony in Burkina Faso, West Africa, using the Illumina GAIIx next generation sequencing platform. Methodology/Principal Findings: We assembled short Illumina reads de novo using a novel approach involving iterative de novo assemblies and ‘‘target-based’’ contig clustering. We then selected a conservative set of 15,527 contigs through comparisons to four Dipt