de novo transcriptome of safflower and the identification of putative genes for oleosin and the biosynthesis of flavonoids新创转录组的红花和公认的基因的识别oleosin和类黄酮的生物合成.pdf

De Novo Transcriptome of Safflower and the Identification of Putative Genes for Oleosin and the Biosynthesis of Flavonoids 1,2. 1. 1 1,2 1 1 1 Haiyan Li , Yuanyuan Dong , Jing Yang , Xiuming Liu , Yanfang Wang , Na Yao , Lili Guan , Nan 1 1,3 1 Wang , Jinyu Wu *, Xiaokun Li * 1 Ministry of Education Engineering Research Center of Bioreactor and Pharmaceutical Development, Jilin Agricultural University, Changchun, Jilin, China, 2 College of Life Sciences, Jilin Agricultural University, Changchun, Jilin, China, 3 Institute of Genomic Medicine, Wenzhou Medical College, Wenzhou, China Abstract Safflower (Carthamus tinctorius L.) is one of the most extensively used oil crops in the world. However, little is known about how its compounds are synthesized at the genetic level. In this study, Solexa-based deep sequencing on seed, leaf and petal of safflower produced a de novo transcriptome consisting of 153,769 unigenes. We annotated 82,916 of the unigenes with gene annotation and assigned functional terms and specific pathways to a subset of them. Metabolic pathway analysis revealed that 23 unigenes were predicted to be responsible for the biosynthesis of flavonoids and 8 were characterized as seed-specific oleosins. In addition, a large number of differentially expressed unigenes, for example, those annotated as participating in anthocyanin and chalcone synthesis, were predicted to be involved in flavonoid biosynthesis pathways. In conclusion, the de novo transcriptome investigation of the unique transcripts provided candidate gene resources for studying oleosin-coding genes and for investigating genes related to flavonoid bi