De novo transcriptome of the desert beetle Microdera punctipennis.pdf
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De novo transcriptome of the desert beetle Microdera punctipennis
(Coleoptera: Tenebrionidae) using illumina RNA-seq technology
Xueying Lu ? Jieqiong Li ? Jianhuan Yang ?
Xiaoning Liu ? Ji Ma
Received: 12 September 2013 / Accepted: 15 July 2014 / Published online: 21 August 2014
The Author(s) 2014. This article is published with open access at S
Abstract Insects in Tenebrionidae have unique stress
adaptations that allow them to survive temperature
extremes. We report here a gene expression profiling of
Microdera punctipennis, a beetle in desert region, to gain a
global view of its environmental adaptations. A total of
48,158,004 reads were obtained by transcriptome
sequencing, and the de novo assembly yielded 56,348
unigenes with an average length of 666 bp. Based on
similarity searches with a cut-off E-value of 10-5 against
two protein sequence databases, 41,109 of the unigenes
(about 72.96 %) were matched to known proteins. An in-
depth analysis of the data revealed a large number of genes
were associated with environmental stress, including genes
that encode heat shock proteins, antifreeze proteins, and
enzymes such as chitinase, trehalose, and trehalose-6-
phosphate synthase. This study generated a substantial
number of M. punctipennis transcript sequences that can be
used to discover novel genes associated with stress adap-
tation. These sequences are a valuable resource for future
studies of the desert beetle and other insects in Tenebri-
onidae. Transcriptome analysis based on Illumina paired-
end sequencing is a powerful approach for gene discovery
and molecular marker development for non-model species.
Keywords Microdera punctipennis Transcriptome
Illumina sequencing Heat shock protein Antifreeze
protein
Introduction
Deserts are among the most hostile habitats on earth. In
summer, it is extremely hot during daytime, while in the
depth of the winter’s night, it is surprisingly cold, as well as
extremely dry in some seasons. Under these extreme con-
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