applying harmonic optical microscopy for spatial alignment of atrial collagen fibers应用谐波光学显微镜心房胶原纤维的空间排列.pdf

Applying Harmonic Optical Microscopy for Spatial Alignment of Atrial Collagen Fibers 1. 2. 3 4 5 5 Yu-Wei Chiu , Men Tzung Lo , Ming-Rung Tsai , Yi-Chung Chang , Rong-Bin Hsu , Hsu-Yu Yu , Chi-Kuang Sun3, Yi-Lwun Ho6,7* 1 Division of Cardiology, Department of Internal Medicine, Far-Eastern Memorial Hospital, Taipei, Taiwan, 2 Research Center for Adaptive Data Analysis, National Central University, Taoyuan, Taiwan, 3 Department of Electrical Engineering, Graduate Institute of Photonics and Optoelectronics, National Taiwan University, Taipei, Taiwan, 4 Graduate Institute of Communication Engineering, National Taiwan University, Taipei, Taiwan, 5 Department of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 6 Graduate Institute of Clinical Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan, 7 Division of Cardiology, Department of Internal Medicine, National Taiwan University Hospital and National Taiwan University College of Medicine, Taipei, Taiwan Abstract Background: Atrial fibrosis creates a vulnerable tissue for atrial fibrillation (AF), but the spatial disarray of collagen fibers underlying atrial fibrosis is not fully elucidated. Objective: This study hypothesizes that harmonics optical microscopy can illuminate the spatial mal-alignment of collagen fibers in AF via a layer-by-layer approach. Patients and Methods: Atrial tissues taken from patients who underwent open-heart surgery were examined by harmonics optical microscopy. Using the two-dimensional Fourier transformation method, a spectral-energy description of image te