Topic 3a Light microscopy University of （话题3大学光学显微镜）.pdf

Optical Microscopy Optical Microscopy Philip D. Rack Assistant Professor University of Tennessee 603 Dougherty Hall prack@ Acknowledgement: This lecture was generated by Professor James Fitz-Gerald at the University of Virginia. University of Tennessee, Dept. of Materials Science and Engineering Optical Microscopy 1.0 Introduction and History • 1.1 Brief Review of Light Physics • 1.2 Characteristic Information 2.0 Basic Principles • 2.1 Ray Optics of the Optical Microscope • 2.2 Summary 3.0 Instrumentation • 3.1 Sample Prep • 3.2 Measurement Systems and Types 4.0 Examples 5.0 Correct Presentation of Results • 5.1 Publication • 5.2 Presentation University of Tennessee, Dept. of Materials Science and Engineering 1.0 Introduction and History • The story of the first compound (more than 1 lens) microscope is an interesting one. Much is unknown, yet many things are known. • Credit for the first microscope is usually given to Zacharias Jansen, in Middleburg, Holland, around the year 1595. Since Zacharias was very young at that time, its possible that his father Hans made the first one, but young Zach took over the production. • Details about these first Jansen microscopes are not clear, but there is some evidence which allows us to make some good guesses about them. • The above early microscope found in Middleburg, Holland, corresponds to our expectations of the Jansen microscopes. University of Tennessee, Dept. of Materials Science and Engineering 1.2 Characteristic Information • Morphology, Size • Transparency or Opacity • Color (reflected and transmitted) • Refractive Indices • Dispersion of Refractive Indices • Pleochroism • Crystal System • Birefringence • Extinction Angle • Fluore