systemic signature of the lung response to respiratory syncytial virus infection系统性的签名呼吸道合胞病毒感染的肺反应.pdf

Systemic Signature of the Lung Response to Respiratory Syncytial Virus Infection 1 1,3 1 2 Jeroen L. A. Pennings *, Annemieke Schuurhof , Hennie M. Hodemaekers , Annemarie Buisman , 2 4 4 3 3 Lia C. G. H. de Rond , Myra N. Widjojoatmodjo , Willem Luytjes , Jan L. L. Kimpen , Louis Bont , Riny Janssen1 1 Laboratory for Health Protection Research, National Institute for Public Health and the Environment, Bilthoven, The Netherlands, 2 Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment, Bilthoven, The Netherlands, 3 Department of Pediatrics, Wilhelmina Children’s Hospital, University Medical Center Utrecht, Utrecht, The Netherlands, 4 Netherlands Vaccine Institute, Bilthoven, The Netherlands Abstract Respiratory Syncytial Virus is a frequent cause of severe bronchiolitis in children. To improve our understanding of systemic host responses to RSV, we compared BALB/c mouse gene expression responses at day 1, 2, and 5 during primary RSV infection in lung, bronchial lymph nodes, and blood. We identified a set of 53 interferon-associated and innate immunity genes that give correlated responses in all three murine tissues. Additionally, we identified blood gene signatures that are indicative of acute infection, secondary immune response, and vaccine-enhanced disease, respectively. Eosinophil- associated ribonucleases were characteristic for the vaccine-enhanced disease blood signature. These results indicate that it may be possible to distinguish protective and unfavorable patient lung respons