synthesis and antiviral bioactivities of 2-aryl- or 2-methyl-3-(substituted- benzalamino)-4(3h)-quinazolinone derivatives的合成和抗病毒生物活性2-arylu2014u2014或者2-methyl-3 -(取代- benzalamino)4(3 h)-quinazolinone衍生品.pdf

Molecules 2007, 12, 2621-2642 molecules ISSN 1420-3049 © 2007 by MDPI /molecules Full Paper Synthesis and Antiviral Bioactivities of 2-Aryl- or 2-Methyl-3-(substituted- Benzalamino)-4(3H)-quinazolinone Derivatives Xingwen Gao, Xuejian Cai, Kai Yan, Baoan Song*, Lili Gao and Zhuo Chen Center for Research and Development of Fine Chemicals, Guizhou University, Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guiyang, 550025, P. R. China. E-mails: chem3333@; alpsjian@163.com; lk81@163.com; glili1983@; gychenzhuo@yahoo. * Author to whom correspondence should be addressed; E-mail: songbaoan22@; Tel.:(+86) 851 3620521, Fax: (+86) 851 3622211. Received: 8 November 2007; in revised form: 17 December 2007 / Accepted: 25 December 2007 / Published: 27 December 2007 Abstract: A simple and general method has been developed for the synthesis of various 4(3H)-quinazolinone derivatives by the treatment of the appropriate 3-amino-2-aryl-4(3H)- quinazolinone with a substituted benzaldehyde in ethanol. The structures of the compounds were characterized by elemental analysis, IR, 1H-NMR and 13C-NMR spectra. The title 2- aryl- or 2-methyl-3-(substituted-benzalamino)-4(3H)-quinazolinone compounds III-1~III- 31 were found to possess moderate to good antiviral activity. Semi-quantitative PCR and Real Time PCR assays were used to ascertain the target of action of compound III-31 against TMV. The studies suggest that III-31 possesses antiviral activity due to induction of up-reg