comparative studies of genome-wide maps of nucleosomes between deletion mutants of elp3 and hos2 genes of saccharomyces cerevisiae比较研究的全基因组地图之间的核小体缺失突变体elp3和hos2基因的酿酒酵母.pdf

Comparative Studies of Genome-Wide Maps of Nucleosomes between Deletion Mutants of elp3 and hos2 Genes of Saccharomyces cerevisiae 1 2 1,3 2 Takashi Matsumoto , Choong-Soo Yun , Hirofumi Yoshikawa , Hiromi Nishida * 1 Genome Research Center, NODAI Research Institute, Tokyo University of Agriculture, Tokyo, Japan, 2 Agricultural Bioinformatics Research Unit, Graduate School of Agricultural and Life Sciences, University of Tokyo, Tokyo, Japan, 3 Department of Bioscience, Tokyo University of Agriculture, Tokyo, Japan Abstract In order to elucidate the influence of histone acetylation upon nucleosomal DNA length and nucleosome position, we compared nucleosome maps of the following three yeast strains; strain BY4741 (control), the elp3 (one of histone acetyltransferase genes) deletion mutant, and the hos2 (one of histone deactylase genes) deletion mutant of Saccharomyces cerevisiae. We sequenced mononucleosomal DNA fragments after treatment with micrococcal nuclease. After mapping the DNA fragments to the genome, we identified the nucleosome positions. We showed that the distributions of the nucleosomal DNA lengths of the control and the hos2 disruptant were similar. On the other hand, the distribution of the nucleosomal DNA lengths of the elp3 disruptant shifted toward shorter than that of the control. It strongly suggests that inhibition of Elp3-induced histone acetylation causes the nucleosomal DNA length reduction. Next, we compared the profiles of nucleosome mapping numbers in gene promoter regions between the control and the disruptant. We detected 24 genes with low conservation level of nucleosome positio