《功能基因组学》第四节课突变体是研究基因功能的基础.pptx

功能基因组学FunctionalGenomics

总结：突变体是研究基因功能的基础突变体的创制方法：物理化学方法（γ-射线、快中子、EMS诱变）生物学方法（Ac-Ds转座子、T-DNA标签）T-DNA标签——T-DNA插入、激活标签、启动子诱捕等基因克隆的方法：同源克隆图位克隆mutmap

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butyieldissocomplextrait…

TillernumberSeednumberperpanicleGrainweightRiceyielddeterminingfactorsRiceyield＝tillernumberXseednumberperpanicleXgrainweight

ThreemethodologiesfordissectingthecomplextraitsMutants–genefunctionsQTLcloning–naturalvariationwithmajoreffectGWAS–naturalvariationswithmajor/minoreffects

Mutants

actin1promoterGUSnptIIubi1stIntronRBLBCaMVterubipromoterCaMVterMutantscreeningAlargescaleofT-DNAtaggingmutantsarephenotypedinthefield.Maetal(2009)JGenetGenomics.36:267

AnumberofmutantswithalteredagronomictraitsWT dep2WT dep2ABCWT Bg3GrainsizePlantMol.Biol.(2009)70:219PLoSGenet.(2011)7:e1002196PlantCellEnviron.(2015)38:800PNAS(2015)112:11102NaturePlants(2015)2:15195TillernumberPlantJ.(2009)58:803PlantCell(2012)24:2562PlantCell(2014)26:4376PlantCell(2015)28:833SeednumberperpanicleCellRes.(2010)20:838PlantCell(2012)24:3235

Phenotypeofdlt(dwarfandlow-tillering)

DLTisanovelcomponentplayingimportantpositiverolesinBRsignalingTongetal(2009)PlantJ.58:803

1-61-3OverexpressionofDLTpromotesricetillering2-5ZH11WT1-11-21-31-41-51-61-72-12-32-42-5RelativeDLTmRNAamount0200400600800LineWT1_11_21_31_41_51_61_72_12_32_42_5DLTmRNA0113.83.71111.5502.4404.20.8586.6339.90.935.4Pheno-typeNormalMoretillersNormalRollleafNormalRollleafRollleafNormalRollleafRollleafNormalMoretillers

WT12345OverexpressionofDLTpromotesricetillering

BIN2hasmultisubstratesTongandChu(2012)JGenet.Genomics,39:3-9Tongetal(2012)PlantCell24:2562-2577OsGSK2

Plant CellArchitectureelongationDLT GA……BRBRI1-BSKs-BSU1GSK2-BZRsGrain StresssizeGL2……NutritionOthersBRFunctionalspecificitiesforcropimprovementPlantJ.(2009)58:803;PlantCell(2012)2