comparative plasma lipidome between human and cynomolgus monkey are plasma polar lipids good biomarkers for diabetic monkeys比较人类和猕猴猴之间的等离子体lipidome等离子体极性脂质好生物标志物糖尿病猴子.pdf

Comparative Plasma Lipidome between Human and Cynomolgus Monkey: Are Plasma Polar Lipids Good Biomarkers for Diabetic Monkeys? 1,2 4 1 1 3 Guanghou Shui *, Jeffrey William Stebbins , Buu Duyen Lam , Wei Fun Cheong , Sin Man Lam , 4 4 1,3 Francine Gregoire , Jun Kusonoki , Markus R. Wenk 1 Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, 2 Life Sciences Institute, National University of Singapore, Singapore, Singapore, 3 Department of Biological Sciences, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore, 4 Lilly Singapore Center for Drug Discovery Pte Ltd., Singapore, Singapore Abstract Background: Non-human primates (NHP) are now being considered as models for investigating human metabolic diseases including diabetes. Analyses of cholesterol and triglycerides in plasma derived from NHPs can easily be achieved using methods employed in humans. Information pertaining to other lipid species in monkey plasma, however, is lacking and requires comprehensive experimental analysis. Methodologies/Principal Findings: We examined the plasma lipidome from 16 cynomolgus monkey, Macaca fascicularis, using liquid chromatography coupled with mass spectrometry (LC/MS). We established novel analytical approaches, which are based on a simple gradient elution, to quantify polar lipids in plasma including (i) glycerophospholipids (phosphatidylcholine, PC; phosphatidylethanolamine, PE; phosphatidylinositol, PI; phosphatidylglycerol, PG; phosphatidyl- serine, PS; phosphatidic acid, PA); (ii) sphingolipids (sphingomyelin,