2017整理Chapter 4 antibody.ppt

三.Genetic engineered antibody 基因工程抗体 1)Chimeric antibody 人鼠嵌合抗体 2)Reshaped antibody 改型抗体 3) Small molecule antibody(Fab; Fv ; single domain Ab;single chain Ab) 小分子抗体易进入细胞 4) Bispecific antibody 双特异性抗体 5) humanized antibody 人源化抗体 1) human Ig gene+plasmid→E.coli→ expression human antibody 2) Mouse B cell Ab Gene knock out →transfer human Ab gene →B cell+tumor →hybridoma →humanized antibody 6) Phage antibody(筛选CD59抗体肽) 噬菌体抗体 point 本章重点 1. Structure and function of Ab 抗体结构与功能 2. Characteristic of five Ab 五类抗体的特点 3. Conception 、 Preparation and Applications of mAb mAb的概念制备及应用 4. Conception of Ab and CDR Ab及CDR的概念 （3） type （型）: Ig can be divided into ? and ? basis of difference of CL ,2:1 （4) subtype（亚型）: leucine-- OZ（十）arginine-- OZ（一）in ? 190 2、Diversity of Ab Immunogenicity （1）Isotype:CH,CL 同种型—种属型标志 （2）allotype:CH, CL 同种异型---个体型标志 （3）idiotype，Id: 5-6 epitope/V 独特型-自体B 细胞型标志。 anti-idiotype antibody，AId § 3 Function of Antibody 第三节抗体的功能 一、 Specifically binding with antigen  特异结合抗原 利用其特异性用于诊断及防治疾病 Ag-Ab structure,T,PH,charge(构型互补，温度适宜，酸碱度合适，电荷相吸） 二、 Activating complement 激活补体 三、binding to Fc receptor 结合Fc受体 1、opsonization 调理吞噬 2、antibody-dependent cell-mediated cytotoxicity，ADCC 效应 3、 Inducing type I hypersensitivity 介导I型超敏反应 四、 Penetrating Placenta and Mucous Membrane 通过胎盘（IgG）与粘膜（IgA） § 4 Properties every immunoglobulin 第四节 各类Ig的特点 一、IgG “ main army” 主力军 IgG is began to synthesize after 3 months in birth, reach adult level in 3～5 y. 1) long half-life ---23d 2) percent of total serum Ig---75% ～80% 3) Unique through the placenta 4) major anti-infection Ig 5) subclass---IgG1- IgG4 Opsonization ADCC activation of complement Binding with staphylococcus protein A，SPA，for diagnosis Induce II、III type hypersensitivity 二、IgM “ ahead army” 先头兵 1) Most Molecular 900KD,macroglobulin 2)