专业英语生物.ppt

Pm34: a new powdery mildew resistancegene transferredfrom Aegilops tauschii Coss. to common wheat Name:SONGLIli Major:Biological Sciences Powdery mildew is a major fungal disease in wheat growing areas worldwide. Catalogue Plant material Methods Results Discussion Plant materia the powdery mildew resistant germplasm line NC97BGTD7 the powdery mildew susceptible cultivar Saluda Chinese Spring nullitetrasomic Nulli5D-tetra5A and ditelosomic Dt5DL lines a single spore culture isolate method (1)Cross experiments (2)Microsatellite markers analysis (3)The PRC reactions (4)Data analysis Chi-square(x2)tests for goodness-of-fit MAPMAKER\Exp Version3.0b NC97BGTD7 (R) ╳ Saluda(S) ↓ shortened NCD7(R) ╳ Saluda(S) ↓ F1 hybrid ╳ F1 hybrid ↓ F2 ╳ F2 ↓ F2:3 ↓ F2:4 (①in the greenhouse ② in the field the F2:3 lines grew in 2002-2003 the F2:4 lines grew in 2003-2004) result Greenhouse evaluations Table 1 Segregation ratios for powdery mildew reaction of F2 derived families from the NCD7 X Saluda cross result The phenotypic classi Wcation of the 10 F2:3lines is shown in Table 1. The chi-square test value was ingood agreement with the expected 1:2:1 ratio of amonogenic trait (21:2:1= 2.47, P = 0.29). The observed pheno-typic classes also Wtted the 1:2:1 ratio (21:2:1= 2.48,P = 0.29) conWrming a single gene controlled resistance Table 2 Segregation ratios for Microsatellite (SSR) markersamong F2 individuals in the NCD7 X Saluda population a AA homozygous for the NCD7 allele b H heterozygous c BB homozygous for the Saluda allele The three mi