高效液相色谱法测定明胶中的六价铬.pdf

现代食品科技 Modern Food Science and Technology 2012, Vol.28, No.8 高效液相色谱法测定明胶中的六价铬 罗东辉，罗海英，吴文海，冼燕萍，郭新东，陈意光，吴玉銮 （广州市质量监督检测研究院, 国家加工食品质量监督检验中心(广州), 广州市食品安全检测技术重点实验室, 广州市食品安全风险动态监测与预警研究中心，广东广州 510110） 摘要：建立了一种测定明胶中六价铬的高效液相色谱法。方法以磷酸氢二钾缓冲溶液超声提取样品中的六价铬，过滤后加入二 苯基卡巴肼与六价铬发生显色反应生成紫红色络合物，通过液相色谱柱分离，可以有效减少杂质干扰，以紫外检测器定性定量测定六 价铬含量。方法定量限为0.8 mg/kg，在添加水平为0.8~50.0 mg/kg 时，回收率大于75%，方法的准确度和精密度均达到检测要求。 关键词：明胶；六价铬；高效液相色谱法 文章篇号：1673-9078(2012)8-1091-1093 Determination of Chromium VI in the Gelatin by High Performance Liquid Chromatography LUO Dong-hui, LUO Hai-ying, WU Wen-hai, XIAN Yan-ping, GUO Xin-dong, CHEN Yi-guang, WU Yu-luan (Guangzhou Quality Supervision and Testing Institute, National Centre for Quality Supervision and Testing of Processed Food (Guangzhou), Guangzhou City Key Laboratory of Detection Technology for Food S afety, Guangzhou City Research Center of Risk Dynamic Detection and Early Warning for Food Safety, Guangzhou Guangdong 510110, China) Abstract: A method for determination of chromium VI in the gelatin by High Performance Liquid Chromatography was developed. Chromium VI was extracted by dipotassium hydrogen phosphate buffer. After filtered, Chromium VI reacted with Diphenylcarbazide to form purple complex. HPLC was applied to the separation and purification of target compound for effectively reducing the disturbance, and the chromiumVI content can be detected selectively by the UV detector. The quantification limit of this method was 0.8 mg/kg. The recovery of chromium VI in gelatin was above 75% in the range of 0.8-50.0 mg/kg. Results indicated that the accuracy and precision met the requirements of the determination of chromium VI content in gelatin. Key word: gelatin, chromium VI, HPLC [1]