c636 aedes albopictus cells have a dysfunctional antiviral rna interference responsec636白纹伊蚊细胞功能失调的抗病毒rna干扰的反应.pdf

C6/36 Aedes albopictus Cells Have a Dysfunctional Antiviral RNA Interference Response 1 2 2 3 3 Doug E. Brackney *, Jaclyn C. Scott , Fumihiko Sagawa , Jimmy E. Woodward , Neil A. Miller , Faye D. 3 3 2 2 2 1 Schilkey , Joann Mudge , Jeffrey Wilusz , Ken E. Olson , Carol D. Blair , Gregory D. Ebel 1 Department of Pathology, University of New Mexico School of Medicine, Albuquerque, New Mexico, United States of America, 2 Arthropod-Borne and Infectious Diseases Laboratory, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, United States of America, 3 National Center for Genome Resources, Santa Fe, New Mexico, United States of America Abstract Mosquitoes rely on RNA interference (RNAi) as their primary defense against viral infections. To this end, the combination of RNAi and invertebrate cell culture systems has become an invaluable tool in studying virus-vector interactions. Nevertheless, a recent study failed to detect an active RNAi response to West Nile virus (WNV) infection in C6/36 (Aedes albopictus) cells, a mosquito cell line frequently used to study arthropod-borne viruses (arboviruses). Therefore, we sought to determine if WNV actively evades the host’s RNAi response or if C6/36 cells have a dysfunctional RNAi pathway. C6/36 and Drosophila melanogaster S2 cells were infected with WNV (Flaviviridae), Sindbis virus (SINV, Togaviridae) and La Crosse virus (LACV, Bunyaviridae) and total RNA recovered from cell lysates. Small RNA (sRNA) libraries were constructed and subjected to high-throughput sequencing. In S2 cells, virus-derived small interfering