subnormal cytokine profile in the tear fluid of keratoconus patients低于正常的细胞因子在圆锥角膜患者的眼泪流体.pdf

Subnormal Cytokine Profile in the Tear Fluid of Keratoconus Patients 1 2 3 4 4 1 Albert S. Jun , Leslie Cope , Caroline Speck , Xiaojun Feng , Seakwoo Lee , Huan Meng , Abdel Hamad5, Shukti Chakravarti 1,4,6* 1 Department of Ophthalmology, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 2 Department of Oncology, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 3 Department of Psychological and Brain Sciences, Johns Hopkins School of Arts and Sciences, Baltimore, Maryland, United States of America, 4 Department of Medicine, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 5 Department of Pathology, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America, 6 Department of Cell Biology, Johns Hopkins School of Medicine, Baltimore, Maryland, United States of America Abstract Keratoconus, historically viewed as a non-inflammatory disease, is an ectatic corneal disorder associated with progressive thinning of the corneal stroma. Recently, a few inflammatory mediators have been reported to be elevated in the tear fluid of keratoconus patients. Consequently, we investigated a wide range of inflammation regulating cytokines in the tears and sera of keratoconus and control subjects. Interleukin (IL)-1b, IL-4, IL-6, IL-10, IL-12, IL-13, IL-17, interferon (IFN)-c, chemokine C-C motif ligand 5 (CCL5) and tumor necrosis factor (TNF)-a were tested in tear samples and sera of keratoconus and control individuals by multiplex immuno-bead assays. Selected cytokines were further tested by standard ELISA on pooled tear samples. All cytokines in the sera were generally low, with no significant changes between ke