the acute environment, rather than t cell subset pre-commitment, regulates expression of the human t cell cytokine amphiregulin严重的环境,而不是t细胞子集预先承诺,调节人类t细胞表达细胞因子amphiregulin.pdf

The Acute Environment, Rather than T Cell Subset Pre- Commitment, Regulates Expression of the Human T Cell Cytokine Amphiregulin 1 1¤ 2 1 Yilin Qi , Darwin J. Operario , Steve N. Georas , Tim R. Mosmann * 1 David H. Smith Center for Vaccine Biology and Immunology, University of Rochester Medical Center, Rochester, New York, United States of America, 2 Department of Medicine, University of Rochester Medical Center, Rochester, New York, United States of America Abstract Cytokine expression patterns of T cells can be regulated by pre-commitment to stable effector phenotypes, further modification of moderately stable phenotypes, and quantitative changes in cytokine production in response to acute signals. We showed previously that the epidermal growth factor family member Amphiregulin is expressed by T cell receptor-activated mouse CD4 T cells, particularly Th2 cells, and helps eliminate helminth infection. Here we report a detailed analysis of the regulation of Amphiregulin expression by human T cell subsets. Signaling through the T cell receptor induced Amphiregulin expression by most or all T cell subsets in human peripheral blood, including naive and memory CD4 and CD8 T cells, Th1 and Th2 in vitro T cell lines, and subsets of memory CD4 T cells expressing several different chemokine receptors and cytokines. In these different T cell types, Amphiregulin synthesis was inhibited by an antagonist of protein kinase A, a downstream component of the cAMP signaling pathway, and