鲤Myf-6 基因的克隆及其表达分析.pdf

2010, 19(9) : 1620 A cta A griculturae Borealioccidental is Sinica * 鲤Myf6 基因的克隆及其表达分析 1 2 1 1 3 1 林亚秋 , 吉 红, 郑玉才 , 邱 翔 , 王树香 , 刘茂琪 ( 1. , 610041; 2. , 712100; 3. , 736202) : ( D anio rerio) Myf6 , ( Cyp rinus carp io) RNA, RTPCR Myf6 ( ORF )720 bp, Gen ank GU339054239 ,90%76%76% 72% , ,59%~ 60% RTPCR Myf6 mRNA , M yf6 , (P 0.05) Myf6 ,( 500 g) (50~ 60 g120~ 130 g) ( P 0. 01) : ; Myf6 ; ; :S917. 4 : A : 2010) Cloning and Expression Analysis of Myf6 Gene of Carp (Cy p rinus carp io ) 1 2 1 1 3 1 LIN Yaqiu , JI Hong , ZHENG Yucai , QIU Xiang , WANG Shuxiang and LIU Maoqi ( 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China; 2. Fishery Sciences Department, College of Animal Science and Technology, Northwest A F University, Yangling Shaanxi 712100, China; 3. Dunhuang City, Gansu Province Qilizhen Petroleum Authority,Dunhuang Gansu 736202, China) Abstract: PCR primers were designed based on Danio rerio Myf6nucleotide sequence. A ORF of 720 bp ( Gen ank accession No. GU339054) of Myf6 gene was obtained with RTPCR using total RNA from muscle of Cyp r inus carp io . T he cDNA encodes a 239 amino acids peptides and the homology of amino acid sequences were 90% , 76%, 76% and 72% compared with that of Danio rerio , Salmo sal ar, S ter nop yg us macr urus and T ak if ugu rubrip es respectively. T he homology of the amino acid se quences was low compared with that of some mam