apoptosis-related gene expression profiles of mouse escs and magscs role of fgf4 and mnda in pluripotent cell responses to genotoxicityescs apoptosis-related老鼠的基因表达谱和magscs fgf4和mnda多能细胞反应基因毒性.pdf

Apoptosis-Related Gene Expression Profiles of Mouse ESCs and maGSCs: Role of Fgf4 and Mnda in Pluripotent Cell Responses to Genotoxicity 1 2 1 1 3 1 Tatjana Khromov , Ralf Dressel , Iliana Siamishi , Jessica Nolte , Lennart Opitz , Wolfgang Engel , D. V. Krishna Pantakani1* 1 Institute of Human Genetics, University of Goettingen, Goettingen, Germany, 2 Department of Cellular and Molecular Immunology, University of Goettingen, Goettingen, Germany, 3 DNA Microarray Facility, University of Goettingen, Goettingen, Germany Abstract Stem cells in the developing embryo proliferate and differentiate while maintaining genomic integrity, failure of which may lead to accumulation of mutations and subsequent damage to the embryo. Embryonic stem cells (ESCs), the in vitro counterpart of embryo stem cells are highly sensitive to genotoxic stress. Defective ESCs undergo either efficient DNA damage repair or apoptosis, thus maintaining genomic integrity. However, the genotoxicity- and apoptosis-related processes in germ-line derived pluripotent cells, multipotent adult germ-line stem cells (maGSCs), are currently unknown. Here, we analyzed the expression of apoptosis-related genes using OligoGEArray in undifferentiated maGSCs and ESCs and identified a similar set of genes expressed in both cell types. We detected the expression of intrinsic, but not extrinsic, apoptotic pathway genes in both cell types. Further, we found that apoptosis-related gene expression patterns of differentiated ESCs and maGSCs are identical to each other. Comparative analysis revealed that several pro- and anti- apoptotic genes are exp