the impact of bisphosphonates on the osteoblast proliferation and collagen gene expression in vitro磷酸盐的影响成骨细胞的增殖和胶原蛋白基因表达在体外.pdf

Koch et al. Head Face Medicine 2010, 6:12 /content/6/1/12 HEAD FACE MEDICINE R E S E A R C H Open Access Research The impact of bisphosphonates on the osteoblast proliferation and Collagen gene expression in vitro †1 †3 1 1 2 Felix Peter Koch , Sareh Said Yekta , Christina Merkel , Thomas Ziebart and Ralf Smeets* Abstract Background: Bisphosphonates are widely used in the clinical treatment of bone diseases with increased bone resorption. In terms of side effects, they are known to be associated with osteonecrosis of the jaw (BONJ). The objective of this study was to evaluate the effect of bisphosphonates on osteoblast proliferation by cell count and gene expression analysis of cyclin D1 in vitro. Furthermore, the gene expression of the extracellular matrix protein collagen type I was evaluated. Nitrogen-containing and non-nitrogen-containing bisphosphonates have been compared on gene expression levels. Methods: Human osteoblast obtained from hip bone were stimulated with zoledronate, ibandronate and clodronate at concentrations of 5 × 10-5M over the experimental periods of 1, 2, 5, 10 and 14 days. At each point in time, the cells were dissolved, the mRNA extracted, and the gene expression level of cyclin D1 and collagen type I were quantified by Real-Time RT-PCR. The gene expression was compared to an unstimulated osteoblast cell culture for control. Results: The proliferation appeared to have been influenced only to a small degree by bisphosphonates. Zolendronate led to a lower cyclin D1 gene expression after 10 days. The collagen