assessing the impacts of experimentally elevated temperature on the biological composition and molecular chaperone gene expression of a reef coral评估的影响实验高温生物成分和分子伴侣蛋白基因表达的一个礁珊瑚.pdf

Assessing the Impacts of Experimentally Elevated Temperature on the Biological Composition and Molecular Chaperone Gene Expression of a Reef Coral 1 1,2 3 1,4 1,2 Anderson B. Mayfield *, Li-Hsueh Wang , Pei-Ciao Tang , Tung-Yung Fan , Yi-Yuong Hsiao , Ching- Lin Tsai5, Chii-Shiarng Chen1,2,5 1 National Museum of Marine Biology and Aquarium, Checheng, Pingtung, Taiwan, R.O.C., 2 Graduate Institute of Marine Biotechnology, National Dong-Hwa University, Checheng, Pingtung, Taiwan, R.O.C., 3 Department of Biology, University of Louisiana, Lafayette, Lafayette, Louisiana, United States of America, 4 Graduate Institute of Biodiversity and Evolution, National Dong-Hwa University, Checheng, Pingtung, Taiwan, R.O.C., 5 Department of Marine Resources, National Sun Yat-Sen University, Kaohsiung, Taiwan, R.O.C. Abstract Due to the potential for increasing ocean temperatures to detrimentally impact reef-building corals, there is an urgent need to better understand not only the coral thermal stress response, but also natural variation in their sub-cellular composition. To address this issue, while simultaneously developing a molecular platform for studying one of the most common Taiwanese reef corals, Seriatopora hystrix, 1,092 cDNA clones were sequenced and characterized. Subsequently, RNA, DNA and protein were extracted sequentially from colonies exposed to elevated (30uC) temperature for 48 hours. From the RNA phase, a heat shock protein-70 (hsp70)-like gene, deemed hsp/c, was identified in the coral host, and expression of this gene was measured with real-time quantitative PCR (qPCR) in both the host anthozoan and endosymbiotic dinoflagellates (genus Symbiodinium). While mRNA levels w