利用消化炉进行大豆分离蛋白中蛋白质含量的快速测定.pdf

/product/777.html 大豆分离蛋白中蛋白质含量的快速测定 杨燕强, 赵发宝 (长治出入境检验检疫局, 山西 长治 046000) : 采用基于经典凯氏定氮法原理设 的KDN-08A 半自动定氮仪对大豆分离蛋白中蛋白质含 量进行快速测定结果经t 检验, 与经典凯氏定氮法测得结果无显著性差异, 且回收率在99. 53% ~ 100. 19%之间表明该方法测定结果准确可靠操作简单快速, 适用于大豆分离蛋白中蛋白质含 量的测定 : 大豆分离蛋白; 蛋白质含量; 快速测定 + :TS 201. 2 1 : B : 1007- 7561( 2007) 05- 59- 03 The rapid determination of protein content in soy protein isolate YANG Ya-gia g, ZHAO Fa-bao ( Cha gzhi E try-Exit I spectio a d Quara ti e Bureau, Cha gzhi Sha xi 046000) Abstract :The rapid determi atio method of protei co te t i soy protei isolate has bee set up by usi g the KDN- 08A automatic- ma ual itroge determi atio apparatus that was desig ed based o the kjeldahl pri - ciple. There is o sig ifica t differe ce betwee the results from above method a d kjeldahl. The recovery rate reached 99. 53%~ 100. 19%. So the rapid method is accurate, rapid, a d simple; a d ca practically used for the determi atio of protei co te t i soy protei isolate. Key words: soy protei isolate; protei co te t; rapid determi atio , 1. 1 , KDN- 08A ; , 0. 0001g; , 90% , 1. 2 ( 1. 84) ; (400g/ L) ; [ 1] ( ) ( 2% ) ; ( 0. 1M) ; ( [2] ) ; ( 0. 1% ) ; , , ( 0. 5% ) ; : 0. 1% + 0. 5% 4~ 6h, ( 1B1) ; ( ) KDN- 08A 1. 3 0. 05%~ 90% , 1. 4 100 , t 1. 5 , 1. 5. 1 , , 2