the effects of overexpression of histamine releasing factor (hrf) in a transgenic mouse model超表达的影响的组胺释放因子(hrf)转基因小鼠模型.pdf

The Effects of Overexpression of Histamine Releasing Factor (HRF) in a Transgenic Mouse Model 2 3 1 1 1 1 Yueh-Chiao Yeh , Liping Xie , Jacqueline M. Langdon , Allen C. Myers , Sun-Young Oh , Zhou Zhu , Susan M. MacDonald4* 1 Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, United States of America, 2 Graduate Institute of Natural Healing Sciences, Nanhua University, Chiayi, Taiwan, 3 Aurora Sinai Medical Center, Milwaukee, Wisconsin, United States of America, 4 The Johns Hopkins Asthma and Allergy Center, Baltimore, Maryland, United States of America Abstract Background: Asthma is a disease that affects all ages, races and ethnic groups. Its incidence is increasing both in Westernized countries and underdeveloped countries. It involves inflammation, genetics and environment and therefore, proteins that exacerbate the asthmatic, allergic phenotype are important. Our laboratory purified and cloned a histamine releasing factor (HRF) that was a complete stimulus for histamine and IL-4 secretion from a subpopulation of allergic donors’ basophils. Throughout the course of studying HRF, it was uncovered that HRF enhances or primes histamine release and IL- 13 production from all anti-IgE antibody stimulated basophils. In order to further delineate the biology of HRF, we generated a mouse model. Methodology/Principal Findings: We constructed an inducible transgenic mouse model with HRF targeted to lung ¨ epithelial cells, via the Clara cells. In antigen