stem cell factor and granulocyte colony-stimulating factor reduce β-amyloid deposits in the brains of appps1 transgenic mice干细胞因子和粒细胞集落刺激因子减少β-amyloid存款appps1转基因小鼠的大脑中.pdf

Li et al. Alzheimer ’s Research Therapy 2011, 3:8 /content/3/2/8 RESEARCH Open Access Stem cell factor and granulocyte colony- stimulating factor reduce b-amyloid deposits in the brains of APP/PS1 transgenic mice 1 1 1 2 1,3 1,4* Bin Li , Maria E Gonzalez-Toledo , Chun-Shu Piao , Allen Gu , Roger E Kelley and Li-Ru Zhao Abstract Introduction: Alzheimer’s disease (AD) is widely recognized as a serious public health problem and heavy financial burden. Currently, there is no treatment that can delay or stop the progressive brain damage in AD. Recently, we demonstrated that stem cell factor (SCF) in combination with granulocyte colony-stimulating factor (G-CSF) (SCF +G-CSF) has therapeutic effects on chronic stroke. The purpose of the present study is to determine whether SCF +G-CSF can reduce the burden of b-amyloid deposits in a mouse model of AD. Methods: APP/PS1 transgenic mice were used as the model of AD. To track bone marrow-derived cells in the brain, the bone marrow of the APP/PS1 mice was replaced with the bone marrow from mice expressing green fluorescent protein (GFP). Six weeks after bone marrow transplantation, mice were randomly divided into a saline control group and a SCF+G-CSF-treated group. SCF in combination with G-CSF was administered subcutaneously for 12 days. Circulating bone marrow stem cells (CD117+ cells) were quantified 1 day after the final injection. Nine months after treatment, at the age of 18 months, mice were sacrificed. Brain sections were processed for immunohistochemistry to identify b-amyloid deposits and GFP expressing bone marrow-derived microglia in the brain. Results: Systemic administration of SCF+G-CSF to APP/PS1 transgenic mice leads to long-t