systematic deletion of homeobox genes in podospora anserina uncovers their roles in shaping the fruiting body系统删除同源框基因柄孢壳菌属anserina揭示他们的角色在形成子实体.pdf

Systematic Deletion of Homeobox Genes in Podospora anserina Uncovers Their Roles in Shaping the Fruiting Body 1,2 ´ 1,2¤ ´ ´ 1 1,2,3 Evelyne Coppin *, Veronique Berteaux-Lecellier , Frederique Bidard , Sylvain Brun , ¨ 1,2,4 1 ¨ 1 1,2,3 1 Gwenael Ruprich-Robert , Eric Espagne , Jinane Aıt-Benkhali , Anne Goarin , Audrey Nesseir , Sara Planamente1, Robert Debuchy 1,2, Philippe Silar 1,2,3* ´ 1 Universite Paris-Sud, Institut de Genetique et Microbiologie UMR8621, Orsay, France, 2 CNRS, Institut de Genetique et Microbiologie UMR8621, Orsay, France, ´ ´ ´ ´ 3 Universite Paris Diderot, Sorbonne Paris Cite, UFR des Sciences du Vivant, Paris, France, 4 Universite Paris Descartes, Sorbonne Paris Cite, Paris, France Abstract Higher fungi, which comprise ascomycetes and basidiomycetes, play major roles in the biosphere. Their evolutionary success may be due to the extended dikaryotic stage of their life cycle, which is the basis for their scientific name: the Dikarya. Dikaryosis is maintained by similar structures, the clamp in basidiomycetes and the crozier in ascomycetes. Homeodomain transcription factors are required for clamp formation in all basidiomycetes studied. We identified all the homeobox genes in the filamentous ascomycete fungus Podospora anserina and constructed deletion mutants f