蜜环菌菌索多糖延缓秀丽隐杆线虫衰老机制研究-天津中草药杂志社.pdf

中草药 Chinese Traditional and Herbal Drugs 第 44 卷 第 4 期 2013 年 2 月 • 449 • 蜜环菌菌索多糖延缓秀丽隐杆线虫衰老机制研究 1 1 1* 2 陈亮稳 ，张云侠 ，于 敏 ，王顺昌 1. 安徽大学生命科学学院 安徽大学中药研究与开发重点实验室，安徽 合肥 230039 2. 淮南师范学院 生命科学系，安徽 淮南 232000 摘 要：目的 探讨蜜环菌菌索多糖（AMP ）延缓秀丽隐杆线虫衰老的作用机制。方法 以模式生物秀丽隐杆线虫作为活 体模型，测定其在正常培养条件下的生存期与后代平均数量，氧化应激条件下的存活率和热休克蛋白 16.2 （HSP-16.2 ）、超 氧化物歧化酶-3 （SOD-3 ）的表达。结果 在正常培养条件下，蜜环菌菌索多糖（AMP-1 、AMP-2 ）显著延长秀丽隐杆线虫 的生存期，对其生殖力没有损害；在氧化应激条件下，显著提高秀丽隐杆线虫HSP-16.2 和 SOD-3 的表达。结论 提高秀丽 隐杆线虫的氧化应激能力可能是AMP 延缓秀丽隐杆线虫衰老的作用机制。 关键词：蜜环菌菌索多糖；抗衰老；秀丽隐杆线虫；热休克蛋白；超氧化物歧化酶-3 中图分类号：R282.710.5 ；R977.9 文献标志码：A 文章编号：0253 - 2670(2013)04 - 0449 - 05 DOI: 10.7501/j.issn.0253-2670.2013.04.015 Anti-aging mechanism of polysaccharide from rhizomorph of Armillaria mellea in Caenorhabditis elegans 1 1 1 2 CHEN Liang-wen , ZHANG Yun-xia , YU Min , WANG Shun-chang 1. Key Laboratory of Chinese Traditional Medicine Research and Development of Anhui University, School of Life Science, Anhui University, Hefei 230039, China 2. Department of Life Science, Huainan Normal University, Huainan 232000, China Abstract: Objective To explore the anti-aging mechanism of polysaccharide from rhizomorph of Armillaria mellea (AMP) in Caenorhabditis elegans. Methods With C. elegans as living model, the life span and the average number of offsprings were determined under normal culture conditions; The survival rate under oxidative stress and the expression of heat shock protein-16.2 (HSP-16.2) and superoxide dismutase-3 (SOD-3) were determined. Results Under the normal culture conditions, the life span of C. elegans was significantly extended by AMP-1 and AMP-2 without the damage of the reproductive capacity; The expression of HS