biomarker discovery in subclinical mycobacterial infections of cattle生物标志物发现亚临床牛分枝杆菌感染.pdf

Biomarker Discovery in Subclinical Mycobacterial Infections of Cattle 1. 1. 1 1 2 Meetu Seth , Elise A. Lamont , Harish K. Janagama , Andrea Widdel , Lucy Vulchanova , Judith R. 3 3 3 1,2 Stabel , W. Ray Waters , Mitchell V. Palmer , Srinand Sreevatsan * 1 Department of Veterinary Population Medicine, University of Minnesota, St Paul, Minnesota, United States of America, 2 Department of Veterinary and Biomedical Sciences, University of Minnesota, St Paul, Minnesota, United States of America, 3 National Animal Disease Center, United States Department of Agriculture, Ames, Iowa, United States of America Abstract Background: Bovine tuberculosis is a highly prevalent infectious disease of cattle worldwide; however, infection in the United States is limited to 0.01% of dairy herds. Thus detection of bovine TB is confounded by high background infection with M. avium subsp. paratuberculosis. The present study addresses variations in the circulating peptidome based on the pathogenesis of two biologically similar mycobacterial diseases of cattle. Methodology/Principal Findings: We hypothesized that serum proteomes of animals in response to either M. bovis or M. paratuberculosis infection will display several commonalities and differences. Sera prospectively collected from animals experimentally infected with either M. bovis or M. paratuberculosis were analyzed using high-resolution proteomics approaches. iTRAQ, a liquid chromatography and tandem mass spectrometry approach, was used to simultaneously identify and quantify peptides