solar disinfection of mods mycobacterial cultures in resource-poor settings太阳能消毒的插件分枝杆菌文化在资源贫乏的环境中.pdf

Solar Disinfection of MODS Mycobacterial Cultures in Resource-Poor Settings 1 2 1,2,3 Ruvandhi Nathavitharana *, Jorge Coronel , David A. J. Moore 1 Department of Infectious Diseases and Immunity and Wellcome Centre for Clinical Tropical Medicine, Faculty of Medicine, Imperial College London, ´ ´ London, United Kingdom, 2 Laboratorio de Investigacion de Enfermedades Infecciosas, Universidad Peruana Cayetano Heredia, San Martın de Porras, ´ ´ ´ ´ Lima, Peru, 3 Asociacion Benefica PRISMA, San Miguel, Lima, Peru Introduction. Safe disposal of TB culture material in which the infectious burden of clinical samples has been greatly amplified is an important challenge in resource-limited settings. The bactericidal capacity of solar cookers has been demonstrated previously for conventional bacteria and contaminated clinical waste. We investigated the use of a simple solar cooker for the sterilization of mycobacterial broth cultures from the microscopic observation drug susceptibility assay (MODS). Methods. Simulated TB culture materials were prepared by inoculating 24-well MODS plates with 500 mL of a known concentration of Mycobacterium bovis BCG. In a series of experiments, samples were simultaneously placed inside a box-type solar cooker and control box and removed at timepoints between 15 minutes and 6 hours. Quantitative cultures were performed using retrieved samples to determine sterilization effect. Results. All cultures from the control box were positive at or within 1–4 logs of inoculation