antitumor activity of emodin against pancreatic cancer depends on its dual role promotion of apoptosis and suppression of angiogenesis抗肿瘤活性的大黄素对胰腺癌取决于其双重作用促进细胞凋亡和抑制血管生成.pdf

Antitumor Activity of Emodin against Pancreatic Cancer Depends on Its Dual Role: Promotion of Apoptosis and Suppression of Angiogenesis 1,3 . 2. 3. 1 3 3 Sheng-Zhang Lin * , Wei-Tian Wei , Hui Chen , Kang-Jie Chen , Hong-Fei Tong , Zhao-Hong Wang , 3 3 3 3 Zhong-Lin Ni , Hai-Bin Liu , Hong-Chun Guo , Dian-Lei Liu 1 Department of Hepato-biliary-pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, China, 2 Department of Oncological Surgery, Zhejiang Cancer Hospital, Hangzhou, China, 3 Department of Hepato-biliary-pancreatic Surgery, Second Affiliated Hospital of Wenzhou Medical College, Zhejiang, China Abstract Background: Emodin has been showed to induce apoptosis of pancreatic cancer cells and inhibit tumor growth in our previous studies. This study was designed to investigate whether emodin could inhibit the angiogenesis of pancreatic cancer tissues and its mechanism. Methodology/Principal Finding: In accordance with our previous study, emodin inhibited pancreatic cancer cell growth, induced apoptosis, and enhanced the anti-tumor effect of gemcitabine on pancreatic caner cells in vitro and in vivo by inhibiting the activity of NF-kB. Here, for the first time, we demonstrated that emodin inhibited tumor angiogenesis in vitro and in implanted pancreatic cancer tissues, decreased the expression of angiogenesis-associated factors (NF-kB and its regulated factors VEGF, MMP-2, MMP-9, and eNOS), and reduced eNOS phosphorylation, as evidenced by both