多重PCR技术在食品微生物检测中的应用进展.pdf

7 010 37 10 PCR , , , ( , 610041) : ( polymerase chain reaction, PCR) , PCR , , PCR , : PCR; ; : Q78 : A : 1671-7 36( 010) 10-007 -04 ( polymerase chain reaction, PCR) , ; , 4~ 7 d PCR , , , , 1 PCR , ( polymerase chain reaction, PCR) , DNA : 010-05- 13 , DNA , DNA : ( 1984- ) , , , , : PCR 3 DNA DNA, : ( 1964- ) , , , E-mail: stang01@ 1 6. com , DNA , 4 al biases[ J] . Nucleic Acids Res, 1994, ( 13) : 437~ 446. evolution. Int Rev Cytol, 199 , 141: 173~ 16. 10 Wolstenholme D R. Animal mitochondrial DNA: structure and Codon Optimizationoff at-1 Gene from Caenorhabdit is el eg an 1 1 1 1 DU Xin-hua , ZHAO Zh-i hui , YU Hao , LI Jun-ya , GA O Xue , XU Shang-zhong ( 1. Key Laboratory of Farm Animal Genet ic Resources and Utilization of M inistry of Agriculture, Inst itute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China; . H usbandry and Veterinary College, Jilin University , Changchun 13006 , China) Abstract:The objective of this study was to optimize the fa-t 1 condon from Cae nor h ad it is e leg an . In this study, on the ba- sis of no changing the am ino acid sequence of f a-t 1 gene, we optimized t he condon of f a-t 1 gene based on the codon preference of bovine muscle protein gene, in order to makef a-t 1 gene had high-level expression in catt le muscle. It could make a prepara- t ion for the production of fa-t 1 tran