丹酚酸B盐对转化生长因子_1刺激肝星状细胞活化与胞内信号转导的作用.pdf

2002 9 25 82 18 　Natl Med J C ina, September 25, 2002, Vol 82, No.18 ·1267 · ·· B -β1 刘成海　刘平　胡义扬　朱大元 【】　　B (SA-B)。　 (HSC), 1、4、7 d, 、3 。100 pmol/L β1(TGF-β1), α- 3 。TGF-β1 HSC , [ H] 0.1 μmol/L ～ 1 mmol/L SA-B , ;Western Nort ern SA-B TGF-β1 HSC 、α- ;,Western SA-B TGF-β1HSC Smad2/3 、。 　TGF-β1 HSC , 128.6%、207.3% 188.2%, HSC TGF-β1 。0.1 mmol/L ～ 1 mmol/L SA-B ,0.1 μmol/L～ 10 μmol/L SA-B , 3 , [ H]76%、60.1%47.8%。 1 μmol/L ～ 10 μmol/L SA-B TGF-β1HSC ( 68.6% 56.1%), α- , I 。0.1 μmol/L ～ 10 μmol/L SA-B Smad2/3 , Smad2 。　SA- B TGF-β1 HSC , TGF-β1 HSC , SA-B 。 【】　;　;　, -β;　 Effects of salvianolic acid- B on TGF-β1 stimulated hepatic stellate cell activation and its intracellular ＊ ＊ signaling　LIU Chenghai , LIU Ping, HU Yiyang, ZHU Dayuan.　 Institute of Liver Diseases, Shanghai University of Traditional Chinese Medicine, Shanghai2000 2, China 【Abstract 】　Objective　To investigate epatic stellate cells (HSC)responses at different differentiation stages on transforming growt factor-β1, and to elucidate t e mec anisms of salvianolic acid -B (SA-B), a water soluble compound from Salvia miltiorrhiza, against epatic fibrosis, relating to interference wit TGF-β1 stimulated HSC activation and intracellular signal transduction via Smads.Methods　HSC was isolated from rat by in situ perfusion of liver and 8.2%nycondenz gradient centrifugation, and primarily cultured on uncoated plastic for 1 d, 4 d and 7 d respectively, w ic represented quiescent, intermediate and activated p enotypes.T e cells were stimulated wit 100 pmol/L TGF-β1, cell p enotypeswere observed under inverted microscope, α-actin expression 3 was c