Acceleration of diabetic wound healing with chitosan-crosslinked collagen sponge.pdf

Available online at 08) 190–204 /locate/lifescieLife Sciences 82 (20Acceleration of diabetic wound healing with chitosan-crosslinked collagen sponge containing recombinant human acidic fibroblast growth factor in healing-impaired STZ diabetic rats Wei Wang a,b, Shaoqiang Lin a, Yechen Xiao c, Yadong Huang d, Yi Tan a,e, Lu Cai a,e,?, Xiaokun Li a,b,c,d,? a The Chinese-American Research Institute for Diabetic Complications, School of Pharmaceutical Sciences, Wenzhou Medical College, China b Key Laboratory of Biotechnology Pharmaceutical Engineering, Wenzhou Medical College, China c Engineering Research Center of Bioreactor and Pharmaceutical Development, Ministry of Education, Jilin Agricultural University, Changchun, China d National Engineering Research Center for Gene Medicine, Jinan University, Guangzhou, China e The Departments of Medicine, Radiation Oncology, and Pharmacology and Toxicology, The University of Louisville, Louisville, USA Received 6 September 2007; accepted 2 November 2007Abstract In order to develop a better wound-dressing to enhance diabetic wound healing, we have examined the biochemical and biophysical features of chitosan-crosslinked collagen sponge (CCCS) and pre-clinically evaluated the CCCS containing recombinant human acidic fibroblast growth factor (CCCS/FGF) in accelerating diabetic wound healing as compared to collagen sponge alone and FGF alone. Collagen crosslinked with chitosan showed several advantages required for wound dressing, including the uniform and porous ultrastructure, less water-imbibition, small interval porosity, high resistance to collagenase digestion and slow release of FGF from CCCS/FGF. Therapeutic effect of the new wound-dressing containing FGF (i.e.: CCCS/FGF) on diabetic wound healing was examined in type 1 diabetic rat model in which hyperglycemia was induced by single dose of streptozotocin (STZ) and persisted for two months. The CCCS/FGF provided the most efficiently therapeutic effect among various treatm