stabilization of the single-chain fragment variable by an interdomain disulfide bond and its effect on antibody affinity稳定的单链片段interdomain变量的二硫键对抗体亲和力及其影响.pdf

Int. J. Mol. Sci. 2011, 12, 1-11; doi:10.3390/ijm OPEN ACCESS International Journal of Molecular Sciences ISSN 1422-0067 /journal/ijms Article Stabilization of the Single-Chain Fragment Variable by an Interdomain Disulfide Bond and Its Effect on Antibody Affinity Jian-Xin Zhao 1, Lian Yang 1,2, Zhen-Nan Gu 3, Hai-Qin Chen 1,2, Feng-Wei Tian 1,2, Yong-Quan Chen 3, Hao Zhang 2 and Wei Chen 1,* 1 School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; E-Mails: jxzhao@ (J.-X.Z.); yanglian27@ (L.Y.); haiqinchen@ (H.-Q.C.); fwtian@ (F.-W.T.) 2 State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China; E-Mail: zhanghao@ (H.Z.) 3 Department of Cancer Biology, School of Medicine, Wake Forest University, Winston Salem, NC 27109, USA; E-Mails: zgu@ (Z.-N.G .); yqchen@ (Y.-Q.C.) * Author to whom correspondence should be addressed; E-Mail: weichen@; Tel./Fax : +86-510 Received: 12 October 2010; in revised form: 8 December 2010 / Accepted: 16 December 2010 / Published: 23 December 2010 Abstract: The interdomain instability of single-chain fragment variable (scFv) might result in intermolecular aggregation and loss of function. In the present study, we stabilized H4—an anti-aflatoxin B (AFB ) scFv—with an interdomain disulfide bond and studied 1 1 the effect of the disulfide