抗RA33抗体的检测及其在类风湿关节炎中的意义（Detection of anti-ra33 antibody and its significance in rheumatoid arthritis）.doc

抗RA33抗体的检测及其在类风湿关节炎中的意义（Detection of anti-ra33 antibody and its significance in rheumatoid arthritis） Detection of anti-ra33 antibody and its significance in rheumatoid arthritis 2010-01-13 liu jianping, yu xiaobi, xuejun, xuejun, xuezhou, xuejun To understand anti RA33 antibody in rheumatoid arthritis (RA) diagnosis, the significance of early diagnosis and prognosis estimation, provide a clue for individualized treatment [1], we use the method of Hassfeld etc of RA 280 cases for testing, the results are as follows. 1 data and methods Study subjects Patient group: 125 cases of RA, 42 for men, 83 for women, age 18 to 76, and 46.1 years on average. Of these, 37 were early. 66 patients who had not used the slow effect of anti-rheumatism or had less than one month of use (hereinafter referred to as the unmedicated). The diagnosis was consistent with the 1987 RA classification standard. Control group: a total of 155 cases, including all kinds of rheumatism, 111 cases, including systemic lupus erythematosus (SLE), 54 cases of ankylosing spondylitis (AS) 47 cases, 10 cases of systemic sclerosis (SSc), diagnostic all conform to the corresponding international standards; The other 44 cases were normal controls. 1.2 method 1.2.1 serum specimen collection, record over the same period in patients with general condition, joint performance, tender joint count, swollen joint, joint index), illness severity classification (Strinbrocker stage), joint performance, the evaluation of physicians and patients to disease activity. The joint performance of the joint was evaluated using 28 joint scoring methods [2], and the progress of the sibling joint X line was evaluated by improving the Sharp scoring standard. 1.2.2 culture collection about 2 x 109 HeLa, extracted by methods such as Hassfeld RA33 antigen, by 12% sodium dodecyl sulfate - polyacrylamide gel electrophoresis (sds) separation, electrophoresis again transferred to nitrocellulose membrane, close after nonspecific sites, wester