biosynthetic studies on water-soluble derivative 5c (dtx5c)生物合成的研究水溶性衍生物5 c(dtx5c).pdf

Mar. Drugs 2012, 10, 2234-2245; doi:10.3390/m OPEN ACCESS Marine Drugs ISSN 1660-3397 /journal/marinedrugs Article Biosynthetic Studies on Water-Soluble Derivative 5c (DTX5c) Tamara S. Vilches 1, Manuel Norte 1,2, Antonio Hernández Daranas 1,3,* and José J. Fernández 1,2,* 1 University Institute for Bio-Organic Chemistry ―Antonio González‖, University of La Laguna, Av. Astrofísico Francisco Sánchez 2, La Laguna 38206, Spain; E-Mails: tvilches@ull.es (T.S.V.); mnorte@ull.es (M.N.) 2 Department of Organic Chemistry, Faculty of Pharmacy, University of La Laguna, Av. Astrofísico Francisco Sánchez s/n, La Laguna 38206, Spain 3 Department of Chemical Engineering and Pharmaceutical Technology, Faculty of Pharmacy, University of La Laguna, Av. Astrofísico Francisco Sánchez s/n, La Laguna 38206, Spain * Authors to whom correspondence should be addressed; E-Mails: adaranas@ull.es (A.H.D.); jjfercas@ull.es (J.J.F.); Tel.: +34-922-318-586; Fax: +34-922-318-571. Received: 10 August 2012; in revised form: 18 September 2012 / Accepted: 20 September 2012 / Published: 12 October 2012 Abstract: The dinoflagellate Prorocentrum belizeanum is responsible for the production of several toxins involved in the red tide phenomenon known as Diarrhetic Shellfish Poisoning (DSP). In this paper we report on the biosynthetic origin of an okadaic acid water-soluble ester derivative, DTX5c, on the basis of the spectroscopical analysis of 13C enriched samples obtained by addition of labelled s